Post-transplant idiopathic non-lupus full-house nephropathy – A case report

We report a case of Idiopathic non-lupus full-house nephropathy (NLFHN) in a 39-year-old male who had a full-house pattern of immunofluorescence study without overt systemic lupus erythematosus after a follow-up of more than 2 years. The incidence of detection of cases of NLFHN is increasing in native kidney biopsy and is critical to report as they have poor clinical outcomes. To the best of our knowledge, it is the first case of post-transplant renal biopsy and needs to be reported to plan the treatment protocol for such transplant patients.

Preliminary phytochemical, microscopic analysis and metabolite profiling of Phoenix pusilla root

Palms are considered as familiar strangers. Various studies reported that the genus Phoenix was mostly cultivated,traditionally used plants as medicine, and also as an ornamental plant. In this present study, preliminary phytochemicalanalysis, the study of microscopical structures and the chemical constituents of Phoenix pusilla root was evaluated.Transverse Section of P. pusilla root was studied by sectioning the two different thickness roots and also powdermicroscopy was carried out. Non-polar, volatile constituents of crude ethanolic extract of root were analyzed byGC–MS. Primary and secondary metabolites quantification revealed that the carbohydrate content and total phenolicsconcentration were higher than the other metabolites. The physicochemical analysis showed the moisture content was3.78 ± 0.54, the ash content value was 2.46 ± 0.95, and the crude fiber content was higher than protein, fat content.Microscopy study showed the presence of unique tracheids. Metabolite profiling revealed the presence of 96 differentconstituents and more than 10 found to have biological importance. Pharmacognostic study is an important step ofresearch which reveals about shelf-life of the drug, adulterations, etc.

Pharmacognostic investigation on roots & leaves extract of Abutilon indicum linn.

Herbal medicines are natural products and their phytoconstitutents vary depending on time and region, processing and storage. Variations in the collection, processing or storage of an herb could impact its efficacy profile. Since prior knowledge regarding appropriate collection and usage of most medicinal plants exists in tradition, it can be used as a guide to quality standardization. The present study is aimed to carry out research work to evaluate Pharmacognostic investigation on Root & Leaf extract of Abutilon indicum. From the literature study it is found that a very little work is done on root & leaf part of plant for setting standardization limits. So, our aim is to find out again various standardization limits according WHO guideline like macroscopic & microscopic study, to find out LOD, ash values, extractive values etc. for root & leaf part of drug with a view to control the quality of crude drug.

Acaricidal activity of synthesized titanium dioxide nanoparticles using Calotropis gigantea against Rhipicephalus microplus and Haemaphysalis bispinosa

OBJECTIVE@#To assess the acaricidal activity of titanium dioxide nanoparticles (TiO2 NPs) synthesized from flower aqueous extract of Calotropis gigantea(C. gigantea) against the larvae of Rhipicephalus (Boophilus) microplus [R. (B.) microplus] and the adult of Haemaphysalis bispinosa (H. bispinosa).@*METHODS@#The lyophilized C. gigantea flower aqueous extract of 50 mg was added with 100 mL of TiO(OH)2 (10 mM) and magnetically stirred for 6 h. Synthesized TiO2 NPs were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and Energy dispersive X-ray spectroscopy (EDX). The synthesised TiO2 NPs were tested against the larvae of R. (B.) microplus and adult of H. bispinosa were exposed to filter paper impregnated method.@*RESULTS@#XRD confirmed the crystalline nature of the nanoparticles with the mean size of 10.52 nm. The functional groups for synthesized TiO2 NPs were 1 405.19, and 1 053.45 cm(-1) for -NH2 bending, primary amines and amides and 1 053.84 and 1 078.45 cm(-1) for C-O. SEM micrographs of the synthesized TiO2 NPs showed the aggregated and spherical in shape. The maximum efficacy was observed in the aqueous flower extract of C. gigantea and synthesized TiO2 NPs against R. (B.) microplus (LC50=24.63 and 5.43 mg/L and r(2)=0.960 and 0.988) and against H. bispinosa (LC50= 35.22 and 9.15 mg/L and r(2) = 0.969 and 0.969), respectively.@*CONCLUSIONS@#The synthesized TiO2 NPs were highly stable and had significant acaricidal activity against the larvae of R. (B.) microplus and adult of H. bispinosa. This study provides the first report of synthesized TiO2 NPs and possessed excellent anti-parasitic activity.

Morphological Studies on Fuchs`Dystrophy

Light andelectron microscopic findings of Fuchs`dystrophy were examined in a corneal button obtained following a penetrating keratoplasty. In light microscopic study, an avascular connective tissue was observed between the epithelial basement membrane and Bowman`s layer. Fibroblast-like cells scattered on the denuded surface of the Descemet`s membrane were found in a flat-mounted specimen examined after alizarine red and tryphan blue staining. A slightly thickened Descemet`s membrane covered with the disorganized endothelium was observed in a electron microscopy. Swollen organelles and vacuoles in the cytoplasm were found as an internal structure of the endothelium.

Malignant Peripheral Nerve Sheath Tumor Arising from Neurofibromatosis

A 65-year-old woman complained of a pedunculated, huge mass on the anterior chest associated with neurofibromatosis type 1. The clinical and histological data showed the possibility of a malignant peripheral nerve sheath tumor. On electron microscopy, we found the characteristic findings of a malignant peripheral nerve sheath tumor, such as the foci of en-tangled cytoplasmic processes, undifferentiated spindle cells having large pleomorphic nuclei with prominent nucleoli, distinct basal cell lamina, rudimentary cell junctions, intermediate filaments and clumps of glycogen in the tumor cells. We, herein, report a case of malignant peripheral nerve sheath tumor arising from neurofibromatosis, studied by electron microscopy.

Morphological Changes in Skeletal Muscle Following Experimental Colchicine Toxicity: Electron Microscopic Study

To investigate the morphological changes in skeletal muscle produced by colchicines toxicity, 42/rats were given a single intraperitoneal dose(0.4mg/Kg) of colchicines, and the animals were killed at daily intervals up to 7 days. The results of light microscopic and electron microscopic observations are summarized as follows; 1) At the light microscopic level, colchicines treated arts show non-inflammatory, nonspecific scattered degeneration or necrosis of myofibers irrespective of location of the skeletal muscle. No significant pathologic changes in peripheral nerve fibers distributed in affected muscles are seen. 2) The general ultrastructural changes are dilation of sarcoplasmic reticulum, swelling and pleomorphism of mitochondria, and appearance of membraneous bodies characterized by a single or multiple concentric layers of membranes in subsarcolemmal zones and intermyofibrillar zones, and focal necrosis or loss of myofibrils. The incidence and severity of these changes show gradual increase and reach maximal peak 3 days after colchicines administration, and then they show gradual decrease. Nerve fibers and motor-end plates show no difference compared with the control. 3) The observations are consistent with the opinion that the muscle weakness or paresis produced by toxic dose of colchicines is not of neuropathic, but of myopathic alterations. 4) The membraneous bodies are classified into the following 3 types;Type I is those bodies outlined by a few concentric membranes with osmiophilic granules, small vesicles, free ribosomes, and mitochondria in central zone. Type II is those outlined by several concentric layers of membranes with osmiophilic granules, small vesicles, free ribosomes, and mitochondria in center. Type III is those outlined by multilayered or thick concentric membranes with osmiophilic granules, small vesicles, free ribosomes, and mitochondria in central zone, frequently with complicated lamellar structures. 5) The skeletal muscle lesions produced by colchicines toxicity may be categorized into those degenerative changes characterized by the appearance of membraneous bodies. And, morphologically, it is presumed that the membraneous bodies are heterogenous in origin, alterations of subcellular structures such as sarcoplasmic reticulum, mitochondria, and lysosome.

Syringocystadenoma Papilliferum: Unusual Location and Electron Microscopic Study

No abstract available.

A Case of Tsutsugamushi Disease / 대한피부과학회지

Tsutsugamushi disease, an infectious disease by Rickettsia(R.) tsntsugamushi, is characterized by eschar, fever and rash. Eschar caused by chigger bite, is pre sent on 47 94% of patients with documented R. tsutsugamushi. We recently noticed one case of R. tsutsugamushi infection. Patient had fever, heaclache, lymphadenopathy, erythematous maculopapules and eschar. The R. tsu tsugamushi was isolated from serum of the patient. Histopathologic findings of eschar show epidermal ulceration overlying a zone of coagulative necrosis of upper dermis and necrotizing vasculitis. E]ectron microscopic findings of endothelial cells of capillary show rickettsia with cell division.

A Case of Familiar Benign Chronic Pemphigus / 대한피부과학회지

A 25-year-old man with familial benign chronic pemphigus presented with a one-year history of a localized pruritic recurrent eruption on his perianal area, Physical examination showed moist, macerated, fissured and scaly patches on erythematous base, A biopsy specimen showed extensive intraepidermal separation containing acantholytic cells. Electron microscopic observation showed widened intercellular space, detachment of the tonofilaments from the desmosomes, and subsequent concentration of the tono filaments around the nucleus. Microvilli were elorigated, thinned and branehed. 1)esmosomes were reduced in number. This case is unique in its clinical location, showing no family tendency.